SCMVM 6, 10, 34 and MCMVA 93, the monoclonal antibodies specific for human cytomegalovirus (HCMV), were used to characterize the antigens localized in the nucleus of HCMV-infected fetal lung fibroblast (FLF). Characterization was performed by the
immunofluorescent staining, the antimetabolite blockage of the temporal expression of viral antigens, and the extraction with the salt and detergent. Immunofluorescent staining revealed that the antigens recognized by SCMVM 6, 34 and MCMVA 93
were
confined to the nucleus of the host cells throughout the infection cycle, however, those recognized by SCMVM 10 appeared in nucleus at first time and migrated to the nuclear membrane and cytoplasm later. Antimetabolite blockage showed that the
antigens
reactive with SCMVM 10 and 34 was early antigens and those recognized by SCMVM 6 and MCMVA 93 was late antigens. By salt extraction with 0.4MNaCl, SCMVM 34 specific antigens were solubilized from nuclear fraction of HCMV-infected FLF, however,
the
MCMVA
93 specific antigens were not extracted even in as high as 1M NaC1. Detergent solubilization test using Triton X-100, Thesit, n-octylglucoside, n-dodecy1-maltoside, octanoy1-N-methylglucamide, CHAPS. CHAPSO, sulfobetaine SB 12, sodium
deoxycholate
and
Triton X-114 showed that SCMVM 6.34 and MCMVA 93 specific antigens were the polar protein associated with but not integrated in the nuclear membrane.
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